Effect of inhibition of Kuppfer cells by gadolinium chloride on ischemia reperfusion injury in rat liver

Objective To investigate the effects of inhibiting Kuppfer cells on rat hepatic ischemia reperfusion injury.Methods Eighty partial hepatic ischemia reperfusion models were prepared and protocol rats were administered with gadolinium chloride,while the control rats with normal saline.Blood pressure,heart rate,AST,TNF-α and IL-1 levels were measured,and morphological changes of liver tissue were observed under a microscope in both groups before and 5 min,1 h and 6 h after reperfusion.ResultsThe levels of TNF-α and IL-1 at 6 h after reperfusion were (0.475± 0.069) μg/L and (0.221± 0.056) μg/L respectively,significantly lower than those in control group (0.831± 0.167) μg/L and (0.335± 0.127) μg/L respectively, P 0.05. The changes in blood pressure,heart rate and AST between two groups also had statistically significant difference (P 0.05). Damages to the ultrastructure in protocol group were significantly alleviated as compared with control group.Conclusion Inhibiting activation of Kupffer cells may attenuate hepatic ischemia reperfusion injury,and Kupffer cells may play an important role in hepatic ischemia reperfusion injury.