Experimental Study on Attenuating the Hepatic Ischemia Reperfusion Injury with Gadolinium Chloride

Objective: To investigate the protection effect of GdCl3 on hepatic ischemia reperfusion injury in rat by inhibiting the activation of Kupffer cells. Methods: Thirty female Wistar rats were divided into 3 experimental groups randomly. The rats were injected NS intravenously 24 and 48 hours preoperatively. Rats in group 1(control group) were administered with the opening and suture of the abdomen with equal NS intravenously. Rats in group 2(I/R) underwent ischemia reperfusion with equal NS intravenously. Rats in group 3(GdCl3+I/R) was performed with both GdCl3 and ischemia reperfusion with 0.2% GdCl3 intravenously. On the third day,the operation of partial hepatic ischemia reperfusion was performed. After 30 minutes of ischemia,the reperfusion was performed for 2 hours. Hepatic tissue was taken out,and was made into 10% tissue homogenate in order to test the LDH enzyme activity. The hepatic cells were extracted to analyze cellular apoptosis and necrosis. The total RNA of hepatic tissue was extracted to detect the expression of IL-10 mRNA by means of RT-PCR. Results: LDH enzyme activity displayed in group 2 was lower than that in control group(P 0.05). The drop of the LDH enzyme activity in group 3 was less than that in control group. Most of the cells in group 2 died. With cell apoptosis only in the initial and late stage,there was less apoptosis in group 3. The expression of IL-10 in group 3 was lower than that in group 2. Conclusion: GdCl3 can inhibit the activation of Kupffer cells,and attenuate hepatic ischemia reperfusion injury significantly.