Study on Optimization of Extraction Technology of Tanshinone IIA from Salvia miltiorrhiza Buhge with Ultrasonic Extraction Method Basea on HPLC

[Objective]The aim was to optimize the extraction condition of tanshinone IIA from Salvia miltiorrhiza Buhge with ultrasonic extraction method.[Method] On the basis of single factor experiment,an optimum extracting technology of tanshinone IIA from S.miltiorrhiza was investigated through the orthogonal experiment under the ultrasonic wave assistance conditions,then the content of tanshinone IIA in extract was analyzed with HPLC method.[Result]The optimum extraction conditions were as follows:at room temperature,ethanol volume fraction 85%,extraction time 30 min,solid-liquid ratio 1∶10,extrating times 4.Under the above conditions,the content of tanshinone IIA in extract was 0.388%.[Conclusion]Extracting tanshinoneIIA with ultrasonic extraction method has characteristics of rapid,efficient,convenient and low energy consumption.It is benefit for producing tanshinoneIIA in large scale.