Study on Extract Process of Tanshinone II_A in Salvia miltiorrhiza

Objective:To explore the best condition to extract tanshinone ⅡA in Salvia mihiorrhiza by ethano1.Method: The optimum condition was determined with L9(34) orthogonal experiment to arrange experiments with extract rate of tanshinone ⅡA as quantitative index.Result: The best extraction process condition was: 8 times of 90 percent alcohol immersed for 30 minutes,reflux extraction was two times with each time of 0.5 hour at 85 ℃.The HPLC result showed that the extraction rate of tanshinone ⅡA was about 91%.Conclusion: Optimized extraction process design is reasonable,reproducible and can increase the reuse of herbs.Salvia mihiorrhiza can be served as a kind of tanshinone resource,which has great value of development and utilization.