Extraction technology optimization of tanshinone II A from Salvia miltiorrhiza Buhge. with ultrasonic extraction method.

[Objective] The aim was to optimize the extraction condition of tanshinone Ⅱ A from Salvia miltiorrhiza Buhge.with ultrasonic extraction method.[Method] On the basis of single factor experiment,an optimum extracting technology of tanshinone Ⅱ A from S.miltiorrhiza was investigated through the orthogonal experiment under the ultrasonic wave assistance conditions,then the content of tanshinone Ⅱ A from S.miltiorrhiza in extract was analyzed with HPLC method.[Result] The optimum extraction conditions were as follows:at room temperature,ethanol volume fraction 85%,extraction time 30min,solid-liquid ratio 1:10,for four times.Under the above conditions,the content of tanshinone Ⅱ A in extract was 0.388%.[Conclusion] Extracting tanshinone Ⅱ A with ultrasonic extraction method has the following characteristics:rapid,efficient,convenient and low energy consumption.It is beneficial to produce tanshinone Ⅱ A in large scale.