2008•Zhonghua zhongyiyao xuekanRequires access

Determination of the Content of Danshensu in Compound Danshen Extract by HPLC

Guo Zhen

Abstract

Objective:To determinate the content of Danshensu in compound Danshen extract with HPLC.Methods:Column:Agilent C_18(250mm×46.5μm);mobilc phase methanol-1% acetic acid;flow rate:1.0mL/min;temperature:room temperature;detective wavelength:280nm.Results:Y=12183X-0.8623(r2=0.9997)linearity range:20.8~208.0μg/mL.The content of danshensu in compund danshen extract was 58.32μg/mL in this group averge.Recovery was 99.74%,RSD=1.42%.Conclusion:This method could be used as the quality control standerd for salvia miltiorrhiza and compund salvia miltiorrhiza preparation.

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Key concepts: Salvia miltiorrhiza, Chromatography, High-performance liquid chromatography, Chemistry, Content determination, Acetic acid, Traditional medicine, Traditional Chinese medicine