Optimization of extraction technique of total alkaloid from Radix Stemona via Response Surface Methodology

【Objective】 The extraction technique of total alkaloid from Radix Stemona was optimized to provide theoretical parameters for its exploiture in agriculture,medicament,foodstuff and so on.【Method】 On the basis of single-factor experiments,the effects of different temperature,time and ethanol weight percent on the extraction yield of total alkaloid from Radix Stemona were investigated with a three-factor Box-Behnken centerunited design.The extraction parameters were optimized with Response Surface Methodology.【Result】 The results indicated that the conditions of 57.76 ℃,75.21% ethanol and heating and refluxing for 13.18 h were optimum,and the yield of total alkaloid could be up to 9.76 mg/g.According to conditions of actual experiment,the optimum extraction parameters were modified at 57.8 ℃ for 13.2 h with ethanol weight percent 75.2%.The validated experiment showed the yield of total alkaloid was 9.63 mg/g under those conditions which was in consistency with the theoretic value.【Conclusion】 The modified extraction parameters were credible and had some practical value.