Optimization of Water Extraction Process of Total Alkaloid from Aconite by Response Surface Methodology

Total alkaloid was extracted from white prepared lateral root of Aconite by acidic water method,which was optimized by response surface designs.Based on the results of one-factor test,response surface methodology with 4 factors and 3 levels was adopted according to box-behnken experimental design principle.Two regression equations for the influence relationships of the extraction rate and four varietals factors were established by response surface experiments.On the basis of regression models,the results with surface were simulated to give optimized result and influencing trends of the main factors on the extraction rate.The optimum extracting conditions are as follows: pH 3.4,liquid-to-solid ratio 11 mg/g,extraction time 4.6 h and temperature 30 ℃.Under these conditions,the extraction rate was 48.5%±1.7%.