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Optimization of Ultrasonic Extraction Technology of Total Alkaloid from Fritillaria thunbergii by Response Surface Methodology

Yue Sun

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Abstract

OBJECTIVE:To optimize the ultrasonic extraction technology of total alkaloid from Fritillaria thunbergii.METHODS:Extracting time,ultrasonic power and liquid-solid ratio were selected as casual factors,based on single-factor experiments,three-factor-three-level Box-Behnken experiment design method was applied,and the extraction rates of total alkaloid were used as response values for response surface methodology(RSM) analysis.RESULTS:The optimum conditions of ultrasonic extraction were as follows:extraction time of 48 min,ultrasonic power of 420 W,and liquid-solid ratio of 6.6(mL:g).Under these conditions,the extraction rate of total alkaloid was 0.168 5%.CONCLUSION:The extraction process optimized by RSM analysis is simple and more convenient for extracting total alkaloid from F.thunbergii.

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What this paper is about

OBJECTIVE:To optimize the ultrasonic extraction technology of total alkaloid from Fritillaria thunbergii.METHODS:Extracting time,ultrasonic power and liquid-solid ratio were selected as casual factors,based on single-factor experiments,three-factor-three-level Box-Behnken experiment design method was applied,and the extraction rates of total alkaloid were used as response values for response surface methodology(RSM) analysis.RESULTS:The optimum conditions of ultrasonic extraction were as follows:extraction time of 48 min,ultrasonic power of 420 W,and liquid-solid ratio of 6.6(mL:g).Under these conditions,the extraction rate of total alkaloid was 0.168 5%.CONCLUSION:The extraction process optimized by RSM analysis is simple and more convenient for extracting total alkaloid from F.thunbergii.

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Available abstract

OBJECTIVE:To optimize the ultrasonic extraction technology of total alkaloid from Fritillaria thunbergii.METHODS:Extracting time,ultrasonic power and liquid-solid ratio were selected as casual factors,based on single-factor experiments,three-factor-three-level Box-Behnken experiment design method was applied,and the extraction rates of total alkaloid were used as response values for response surface methodology(RSM) analysis.RESULTS:The optimum conditions of ultrasonic extraction were as follows:extraction time of 48 min,ultrasonic power of 420 W,and liquid-solid ratio of 6.6(mL:g).Under these conditions,the extraction rate of total alkaloid was 0.168 5%.CONCLUSION:The extraction process optimized by RSM analysis is simple and more convenient for extracting total alkaloid from F.thunbergii.

Key concepts: Response surface methodology, Extraction (chemistry), Chromatography, Ultrasonic sensor, Alkaloid, Materials science, Chemistry, Stereochemistry

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Optimization of Ultrasonic Extraction Technology of Total Alkaloid from Fritillaria thunbergii by Response Surface Methodology — Research Paper | ScholarLens