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Determination of four effective components in Muxiang Shunqi pills by HPLC

Chunyu Li

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Abstract

OBJECTIVE To establish a method for determination of costunolide,dehydrocostuslactone,magnolol and honokiol in Muxiang Shunqi pills by HPLC.METHODSKromasil C18(250 mm×4.6 mm,5.0 μm)was used with the mobile phase consisted of acetonitrile-menthanol-water(50:8:42).The flow rate was 1.0 ml·min-1 with the detection wavelength at 210 nm.RESULTSThe linear range of the method was 0.9996 to 0.9999.The average recovery was 97.5%-100.8% with corresponding RSD of 0.88%-1.77%.CONCLUSIONThe method is specific and repeatable,which can be used for the quality control of Muxiang Shunqi pills.

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What this paper is about

OBJECTIVE To establish a method for determination of costunolide,dehydrocostuslactone,magnolol and honokiol in Muxiang Shunqi pills by HPLC.METHODSKromasil C18(250 mm×4.6 mm,5.0 μm)was used with the mobile phase consisted of acetonitrile-menthanol-water(50:8:42).The flow rate was 1.0 ml·min-1 with the detection wavelength at 210 nm.RESULTSThe linear range of the method was 0.9996 to 0.9999.The average recovery was 97.5%-100.8% with corresponding RSD of 0.88%-1.77%.CONCLUSIONThe method is specific and repeatable,which can be used for the quality control of Muxiang Shunqi pills.

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Available abstract

OBJECTIVE To establish a method for determination of costunolide,dehydrocostuslactone,magnolol and honokiol in Muxiang Shunqi pills by HPLC.METHODSKromasil C18(250 mm×4.6 mm,5.0 μm)was used with the mobile phase consisted of acetonitrile-menthanol-water(50:8:42).The flow rate was 1.0 ml·min-1 with the detection wavelength at 210 nm.RESULTSThe linear range of the method was 0.9996 to 0.9999.The average recovery was 97.5%-100.8% with corresponding RSD of 0.88%-1.77%.CONCLUSIONThe method is specific and repeatable,which can be used for the quality control of Muxiang Shunqi pills.

Key concepts: Chemistry, Magnolol, Honokiol, Chromatography, High-performance liquid chromatography, Linear range, Pill, Detection limit

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