HPLC Fingerprint Analysis for Methanol Extraction of Muxiang Shunqi Pills

Objective:To investigate the fingerprint for methanol extraction of Muxiang Shunqi pills by HPLC-UV.Method:The samples were separated with an Agilent Zorbax Eclipse XDB C18 reversed-phase column(4.6 mm × 150 mm,5 μm) by linear gradient elution using water-acetonitrile as mobile phase;the flow rate was 1 mL.min-1 and the detection wavelength was set at 280 nm,225 nm(rosa banksiae);the column temperature was set at 35 ℃.Muxiang Shunqi pills from different pharmaceutical company were compared and the individual crude drugs were analyzed.Result:Honokiol was linear in the range of 104-520 mg.L-1,Y=70 580X + 1.187 5.r =0.999 7;RSD of all relative retention time and relative peak area achieved good results,the precision test and stability test satisfied the requirements and repeatability test was less than 1% and 3% respectively.Conclusion:The quality of Muxiang Shunqi pills is obviously different;one of the main reasons of such state is the quality of crude drugs.