Qualitative and Quantitative Analysis of Salviaflaside in Prunella vulgaris by TLC and HPLC

Objective:To establish an effective TLC and HPLC method for qualitative and quantitative analysis of salviaflaside in P.vulgaris which can be used to be one of the quality control of P.vulgaris.Method: Salviaflaside was identified by TLC in P.vulgaris and different parts from P.vulgaris;salviaflaside was determined by RP-HPLC.Chromatography conditions were Agilent Eclipse XDB-C18(4.6 mm×250 mm,5μm) column with mobile phase of acetonitrile-1.0% acetic acid(16∶84),UV detection wavelength was set at 319 nm and the column temperature was set at 30 ℃ with the flow rate of 1.0 mL · min-1;the sample injection was 10 μL.Result: The TLC sports developed were fairly clear only in flower spike with no interference;good linearity of salviaflaside was obtained in the range of 0.032 667-0.653 34 μg(r=0.999 9).The recovery of salviaflaside was 99.18% and RSD was 0.25%.The content of salviaflaside was more than 0.1‰.Conclusion: Salviaflaside was characteristic compound in flower spike of P.vulgaris.The method is simple,reliable and accurate,which can be applied as the quantity control method of P.vulgaris.