Research on the determination methods of Mecarbinate

By the methods of HPLC and TLC,the content of Mecarbinate is determinated with reaction endpoint controlled.HPLC method used for Kromasil sum C18 column(150 mm×4.6 mm);mobile phase:0.02% potassium dihydrogen phosphate(pH 3.0)-acetonitrile(65∶ 35),detection wavelength 217 nm,flow rate 1.0 mL/min.TLC method on silica gel GF254 thin layer plate,dichloromethane and methanol(20∶ 1) as developing agent.Results showed that the linear ranges of Mecarbinate was 0.8~8.0 μg,the results of recovery experiment was 98.9 %.Thus determination endpoint by TLC is simple and quick.The method HPLC on Mecarbinate content determination is accurate,simple,stable.The determination method on Mecarbinate by HPLC and TLC is established,which is suitable for the quality controlling of Mecarbinate during the industrial producing process.