Study on the Standard for Quality Control of Liangejiangtang Tablet

Aim To establish a standard for quality control of Liangejiangtang tablet.Methods TLC method was used for qualitative analysis of the materials of the prescription,including Rhizome coptidis,Radix puerapiae and Folium mori.The HPLC method was adopted to establish a method for determination of Berberine Hydrochloride contents in the tablet.The HPLC method was adopted and a C18(250mm×4.6mm,5μm) was used as the stationary phase column.The mobile phase was methanol-0.033mol · L-1 Potassium dihydrogen phosphate solution(30∶ 70) at a flow rate of 1.0ml/min.The detection wavelength was 265nm.The HPLC method was adopted to establish a method for determination of Puerain contents in the tablet.A C18(150mm×4.6mm,5μm) was used as the stationary phase column.The mobile phase was methanol-0.1% Phosphoric Acid solution(11∶ 89) at a flow rate of 1.0ml/min.The detection wavelength was 250nm.Results In TLC,the spots were discernible and apparent with strong proprietary display features.The linearity of Berberine Hydrochloride was excellent over the range of 0.101 4~2.028 0μg(r=0.999 9).The average recovery was 98.4%,RSD=1.08%(n=6).The linearity of Puerain was excellent over the range of 0.084 4 ～1.688μg(r=0.999 8).The average recovery was 98.7%,RSD=1.10%(n=6).Conclusion HPLC and TLC can be used as the standard for quality control of Liangejiangtang tablet.