Extraction of polysaccharide in squid ink by enzymatic method

Using papain to extract squid ink polysaccharide,and discuss the process of protein removal.Experimental results show that the optimal conditions for enzyme is:enzyme dosage 12800 U/g,pH 7.0,hydrolysis temperature 55～65 ℃ and reaction time within the scope of more than 90 min.At this point the content of polysaccharides is 90.36%,polysaccharide's yield is 0.95%,protein extrusion rate was 90.52%.This manuscript also compared with the trichloroacetic acid method,the Sevag method,the combination of enzyme and Sevag method to remove the protein.The results showed that the combination of enzyme and Sevag method can be simply and efficient to remove the protein of polysaccharide in squid ink,polysaccharide without causing too much damage,and the loss rate of polysaccharides is 12.45%.