Application on removing protein method of beer yeast polysaccharides

Sevag and Sevag combined of enzymatic were applied to remove protein in crude beer yeast polysaccharides. The results showed:the volume ratio of chloroform to N- butanol was 5∶1,solution of polysaccharides:Sevag reagent was 5∶1,when it was dealt with 3 times attained the optimum condition,concentration of protein was 6.392mg/mL,polysaccharide loss was 24.612%. Sevag combined of enzymatic,at 60℃,pH7.0,the volume ratio of solution of polysaccharides to enzyme for 3∶1,reacting for 2h,the concentration of protein was 6.86mg/mL,polysaccharide loss was 19.74%.