Optimization of De-protein Process for Polysaccharide of Hippophae rhamnoides

Removing protein is one of the important elements in the process of plant polysaccharide purification.In order to improve the efficiency of polysaccharide purification,the present paper mainly investigated the technology of protein removal from Hippophae rhamnoides polysaccharide,so as to optimize the process of de-protein.Three different methods of removing protein,the acetocaustin method(TCA),the Sevag method and the combination of enzyme with Sevag method,were compared by analyzing the content of protein and polysaccharide content in the poly-saccharide liquor after treated with above approaches.The results showed that,for the method of TCA,5% trichloroacetic acid had the highest efficiency for removing protein and had the minimum loss of polysaccharide.For the method of Sevag,treatment repeatedly for five times had the relatively good efficiency of protein removal and less loss rate of polysaccharide.For the method of enzyme plus Sevag,treatment for twice is the best choice.By comparison,the combination of enzyme with Sevag is the bset among the three methods.In addition,the paper further designed a orthogonal experiment to determine the best condition of deproteinised method of papain,which indicated that the best condition was the papain volume of 5mg/mL,hydrolytic temperature of 60℃ and pH of 6,under which the protein clearance rate reached to 73.58%.