A Primary Study of Loss of Heterozygosity on Chromosome 1 in Glioblastoma

Purpose 31 loci on chromosome 1 were examined to detect LOH in 21 cases of glioblastoma(GBM) in order to locate the deletion areas probably harboring tumor suppressor genes. Methods PCR based microsatellite polymorphism analyses were performed to detect LOH on chromosome 1,fluorescence labeled primers and Perkin Elmer 377 DNA Sequencer were applied. Results 52% informative cases of GBM displayed LOH on chromosome 1.20% of informative loci showed LOH in our series, in which the most frequent LOH were observed at loci D1s218 on 1q24-1q25 and from D1s2785 to D1s2842 on 1q32.3-1q43 .The frequency of LOH at all loci examined on chromosome 1p was less than 30%. Conclusions Chromosome 1p is not involved in the molecular genetic pathogenesis of GBM.Loss of genetic material on chromosome 1q may play an important role on the initiation and progressiion of GBM.The chromosomal regions at loci D1s218 on 1q24-1q25 and from D1s2785 to D1s2842 on 1q32.3-1q43 may harbor several tumor suppressor genes associated with GBM.