A preliminary study of loss of heterozygosity on chromosome 3 in glioblastoma

Objective To detect loss of heterozygosity (LOH) in order to locate the deletion areas probably harboring tumor suppressor genes(TSGs) in glioblastoma (GBM). Methods PCR based microsatellite polymorphism analyses were performed to detect LOH on chromosome 3, fluorescence labeled primers and Perkin Elmer 377 DNA Sequencer were applied. Results 50% informative cases of GBM displayed LOH on chromosome 3. 25.6% of informative loci showed LOH in our series, in which the higher frequent LOH were observed in the chromosomal region from loci D3s1614(42.9%) to D3s1565 on 3q24 27 and at loci D3s1569(35.3%) on 3q22 23 and D3s1289 (33.3%) on 3p14.1 14.3. Conclusion Loss of genetic material on chromosome 3 may play an important role on the initiation and progression of GBM. The chromosomal regions from loci D3s1614 to D3s1565 on 3q24 27 and at loci D3s1569 on 3q22 23 and D3s14.3 may harbor novel tumor suppressor genes associated with GBM.