Inhibition of DADS on the Proliferation of Human Leukemia Cell Line HL-60

Objective To explore the potential therapeutic effect of DADS on human leukemia cell line HL-60. Methods MTT was used to test the cell growth of HL-60, cell cycle and apoptosis of human leukemia cell line HL-60 were detected by flowcytometry (FCM), immunohistochemical staining detected the expression of PCNA. Results DADS significantly inhibited proliferation of HL-60 cell and the inhibiting effects had a dose-and time-dependence.The inhibition fraction of HL-60 cell exposed to 20 mg·L -1 DADS for 72 h was 70.5%, flow cytometry analysis revealed that HL-60 cell treated with DADS significantly increased in the percentage of cells in the G 2/M and S phase, and appearance of sub-G 1 peak in a dose-dependent manner. Immunohistochemical staining revealed that the expression of PCNA HL-60 cell reduced(P0.05),when treated with 10 mg·L -1 DADS.Conclusion DADS can effectively inhibit the proliferation of human leukemia cell line HL-60 cell and the possible mechanisms may be related to G 2/M and S phase arrest and apoptosis induced by DADS.