Effect of Diallyl Disulfide on the Proliferation,Cell Cycle and the Expression of p21 of Human Leukemia Cell Line HL-60

Objective To study the proliferation ,cell cycle and expression of cyclin-dependent kinase inhibitors (CKIs) of human leukemia cell line HL-60 treated with diallyl disulfide (DADS) ,and to investigate the mechanism of DADS to inhibit tumor cell’s growth and induce their G2/M phase arrest. Methods We treated HL-60 cells with varible dose DADS ,detected the proliferation by MTT method and observed cell cycle by flow cytometer ,used Western blot to detect the protein level of p21. Results DADS could inhibit the proliferation of HL-60 ,bring about G2/M phase arrest and up-regulate the level of protein of p21. Conclusions DADS could inhibit proliferation and induce G2/M phase arreston HL-60 cells by up-regulating experession of p21.