STUDY ON ANTIOXIDANT ACTIVITY OF THE HYDROLYZATE OF CORN PROTEIN HYDROLYZED BY ALCALASE 2.4 L

In this paper,alcalase 2.4 L was used to hydrolyze corn protein,the reducing power of the corn protein hydrolyzate prepared under different conditions was studied,and the enzymatic hydrolysis conditions were optimized by using response surface methodology.The optimum enzymatic hydrolysis conditions were as follows: the substrate concentration 2.35%, 3.70%,hydrolysis temperature 55.59 ℃,hydrolysis time 5.12 hours,and pH value 8.67.The reducing power A700nm of the hydrolyzate prepared under the optimum conditions was up to 0.436,which was significantly larger than that of 60 g/mL Vc solution and was close to that of 80 g/mL Vc solution.The results indicated that the hydrolyzates had excellent antioxidant activity and could be applied value in the food industry.