Optimization of Preparation Process of High F-Value Corn Peptide by Microwave-Assisted Enzymatic Hydrolysis

Microwave-assisted enzymatic hydrolysis conditions for preparing high F-value corn peptide were optimized.Corn gluten meal(CGM) was used as the raw material and degreased by supercritical CO2 fluid.Alcalase 2.4L and papain were used to hydrolyze CGM.The optimal alcalase hydrolysis conditions were substrate concentration of 9 g/100 g,alcalasesubstrate ratio of 3 g/100 g,hydrolysis pH of 9.0,microwave power of 300 W and microwave treatment time of 2.5 min.Under the optimal conditions for alcalase,the yield of corn peptide was up to 23.8 g for 100 g CGM.Similarly,the optimal papain hydrolysis conditions were papain-substrate ratio of 4 g/100 g,hydrolysis pH of 7.5,microwave power of 100 W,microwave treatment time of 5.0 min and alkali consumption of 6.22 mL/100 mL.The F value of the corn peptide prepared by microwave-assisted enzymatic hydrolysis was 27.15,which revealed an improvement by 21% when compared with traditional water-bath enzymatic hydrolysis.Moreover,microwave-assisted enzymatic hydrolysis time was shortened.