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2014•China Forestry Science and TechnologyRequires access

Techniques for tissue culture on Lonicera japonica ‘Jinhua 3'

Zhao Xian-ha

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Abstract

Tissue culture techniques for Lonicera japonica‘Jinhua 3'were studied. The results showed that the suitable induction medium was MS + 6-BA 1. 5 mg / L + NAA 0. 1 mg / L. The suitable proliferation medium with the robust and orderly buds was MS + 6-BA 1. 0 mg / L + NAA 0. 2 mg / L,the multiplication coefficient of which was 4. 5 roughly. The suitable rooting medium was 1 /2MS + NAA 0. 2 mg / L + IBA 0. 4 mg / L,the rooting rate of which reached 90%. The transplanting matrix were peat and perlite(Vpeat+ Vperlite= 3∶ 1),and the transplanting survival rate of which was up to 90%.

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What this paper is about

Tissue culture techniques for Lonicera japonica‘Jinhua 3'were studied. The results showed that the suitable induction medium was MS + 6-BA 1. 5 mg / L + NAA 0. 1 mg / L. The suitable proliferation medium with the robust and orderly buds was MS + 6-BA 1. 0 mg / L + NAA 0. 2 mg / L,the multiplication coefficient of which was 4. 5 roughly. The suitable rooting medium was 1 /2MS + NAA 0. 2 mg / L + IBA 0. 4 mg / L,the rooting rate of which reached 90%. The transplanting matrix were peat and perlite(Vpeat+ Vperlite= 3∶ 1),and the transplanting survival rate of which was up to 90%.

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Available abstract

Tissue culture techniques for Lonicera japonica‘Jinhua 3'were studied. The results showed that the suitable induction medium was MS + 6-BA 1. 5 mg / L + NAA 0. 1 mg / L. The suitable proliferation medium with the robust and orderly buds was MS + 6-BA 1. 0 mg / L + NAA 0. 2 mg / L,the multiplication coefficient of which was 4. 5 roughly. The suitable rooting medium was 1 /2MS + NAA 0. 2 mg / L + IBA 0. 4 mg / L,the rooting rate of which reached 90%. The transplanting matrix were peat and perlite(Vpeat+ Vperlite= 3∶ 1),and the transplanting survival rate of which was up to 90%.

Key concepts: Transplanting, Perlite, Japonica, Tissue culture, Botany, Horticulture, Chemistry, Biology

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