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Determination of Paeoniflorin in Shenqijianwei Granule by HPLC

Chunyan Lu

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Abstract

ObjectiveTo establish the determination method of Shenqijianwei Granule.MethodsHPLC was adopted with Kromasil 100-5 C18(4.6 mm×250 mm).The mobile phase corsrsted of acetonitrile:Methanol-water(38∶62).The detection wavelength was at 230 nm.The flow rate was 0.7 ml/min,and the column temperature was at 30℃.ResultsThe linearity of this method was well with a good seperation.The calibration curve was linear(r=0.999)in the range of 0~50μg/ml for Paeoniflorin.The average recovery was 97.90%,RSD=1.186%.The contents of paeoniflorin were 0.0242%,0.0274%,0.02166% in three batch of sample product respectively,and the average content of paeoniflorin was 0.024 43%,ConclusionThe method can be used for the quality control of Shenqijianwei Granule.

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What this paper is about

ObjectiveTo establish the determination method of Shenqijianwei Granule.MethodsHPLC was adopted with Kromasil 100-5 C18(4.6 mm×250 mm).The mobile phase corsrsted of acetonitrile:Methanol-water(38∶62).The detection wavelength was at 230 nm.The flow rate was 0.7 ml/min,and the column temperature was at 30℃.ResultsThe linearity of this method was well with a good seperation.The calibration curve was linear(r=0.999)in the range of 0~50μg/ml for Paeoniflorin.The average recovery was 97.90%,RSD=1.186%.The contents of paeoniflorin were 0.0242%,0.0274%,0.02166% in three batch of sample product respectively,and the average content of paeoniflorin was 0.024 43%,ConclusionThe method can be used for the quality control of Shenqijianwei Granule.

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Available abstract

ObjectiveTo establish the determination method of Shenqijianwei Granule.MethodsHPLC was adopted with Kromasil 100-5 C18(4.6 mm×250 mm).The mobile phase corsrsted of acetonitrile:Methanol-water(38∶62).The detection wavelength was at 230 nm.The flow rate was 0.7 ml/min,and the column temperature was at 30℃.ResultsThe linearity of this method was well with a good seperation.The calibration curve was linear(r=0.999)in the range of 0~50μg/ml for Paeoniflorin.The average recovery was 97.90%,RSD=1.186%.The contents of paeoniflorin were 0.0242%,0.0274%,0.02166% in three batch of sample product respectively,and the average content of paeoniflorin was 0.024 43%,ConclusionThe method can be used for the quality control of Shenqijianwei Granule.

Key concepts: Paeoniflorin, Granule (geology), Chromatography, Calibration curve, Chemistry, High-performance liquid chromatography, Content determination, Acetonitrile

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Determination of Paeoniflorin in Shenqijianwei Granule by HPLC — Research Paper | ScholarLens