Expression of survivin gene in laryngeal carcinoma and its relationship with apoptosis

Objective To study the expression of a novel apoptosis inhibitor,survivin,in laryngeal carcinoma and its relationships with apoptosis and clinical pathologic parameters.Methods The expressions of survivin protein in Hep2 cell line,periodontal ligament cells(PDLC),40 laryngeal carcinoma and 8 normal laryngeal tissue were detected by immunohistochemical staining.The expression of survivin mRNA was detected by RT-PCR.DNA in situ end labelling technique was used to detect apoptosis index(AI).Results Survivin protein expressed in Hep2 cell line and 23 of 40(57.5%) cases of laryngeal carcinoma.In contrast,it didn′t express in PDLC and normal laryngeal tissues.The positive percentage of survivin protein in tumor grade Ⅰ laryngeal carcinoma(33.3%) was strikingly lower than that of grade Ⅱ and Ⅲ laryngeal carcinoma(72.7%,P0.05).The positive percentage of survivin protein in clinical stage Ⅲ and Ⅳ laryngeal carcinoma(76.2%) was strikingly higher than that of grade Ⅰand Ⅱ laryngeal carcinoma(36.8%,P0.05).The positive percentage of survivin protein in laryngeal carcinoma with lymph node metastasis(100%) was strikingly higher than that of laryngeal carcinomas without lymph node metastasis(48.5%,(P0.05,).)AI of survivin protein positive cases(2.93±1.20) was lower than that of negative cases(4.05±1.42,P0.05).Conclusion The results indicate that apoptosis inhibition caused by survivin protein may play a important role in the origin and progression of laryngeal carcinoma.