2004•Chinese Archives of Otolaryngology-head and Neck SurgeryRequires access
Expression and implication of survivin gene in laryngeal carcinoma
Sun Ya-xin, Lianji Wen
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Abstract
OBJECTIVE To investigate the ex-pression of inhibitor apoptosis protien of survivingene and determine the role in the pathogenesisof laryngeal squamous cell carcinoma. METHODSThe expression of survivin gene were analysedby immunohistochemistry SP method in 42 la-ryngeal carcinoma tissues,20 laryngeal kerato-sis tissues,20 tissues adjacent to the cancer,and10 normal laryngeal tissue. RT - PCR was usedto test the survivin mRNA expression in humanlaryngeal squamous cell line Hep-2. RESULTSThe expression of survivin gene was not observedin normal laryngeal tissues. The positive rate ofexpression of survivin protein in laryngeal carcinoma(66.6 %) was significantly higher than that in theadjacent cancer tissues (40 %) and in keratosis oflarynx (35 % ) (P 0.001). But the expression rateof survivin gene was not related to clinical stageand pathological grade and neck lymph nodemetastases(P 0.05).The expression of survivin mRNAwas detected in Hep-2. CONCLUSION The up-regu-lation expression of survivin gene in laryngeal car-cinoma suggested that survivin gene may play arole in the pathway of carcinogensis.[