Influence of Processing on Active Components in Angelica sinensis

OBJECTIVE To compare the changes of active components in Angelica sinensis before and after processing by HPLC/UV.METHODS HPLC-DAD method was applied and the gradient elution mode was applied for determining the contents of the liqustilide and ferulic acid in different processed products of Angelica sinensis. The separation was performed on a Zorbax SB-C18(4.6 mm×250 mm,5 μm)column with the gradient elution mode consisting of acetonitrile and H2O (containing 0.01% phosphoric acid). The column temperature was set up at 25 ℃,the analysis time lasted 60 min and the flow rate was 0.8 mL·min-1.Detection wavelength was 220 nm. RESULTS The contents of components were changed after Angelica sinensis(Oliv.)Diels was processed by different methods.CONCLUSION It is simple, reliable and repeatable.The method can be used for the equality control of Angelica sinensis(Oliv.)Diels and its different processed products. The results have provided a scientific basis for processing Angelica sinensis(Oliv.) Diels.