2004•Chinese Medical Journal of Metallurgical IndustryRequires access

Determination of ferulic acid in angerlia sinensis(Oliv.)diels by using high-performance liquid chromatographic method

Xinhao Li

Abstract

Objective:To determine ferulic acid content in angelia sinensis(Oliv.)diels.Methods:1% Acetic acid was used as solvent for ferulic acid and 40%.Methanol was used as solvent for angerlia sinensis.ARP-HPLC method that determined the ferulic acid content in Angelica Sinensis was set up.The HPLC with BATESIL C 18(4.6× 250 mm,5-Micro)column was used,the Methanol:Tetrahydrofuran:1% Acetic acid=30∶1∶70 as a mobile phase and detection wavelength at 320 nm.Results:A good linearity was obtained from 3.05～ 30.5 μg·ml -1with r=0.9999 for ferulic acid.The average recovery was 98.8%,RSD=1.07%(n=9).Conclusions:HPLC and determinate ferulic acid content in angelia sinensis(Oliv.) diels.The method was proved to be simple,precise,sensitive.

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Key concepts: Ferulic acid, Chromatography, High-performance liquid chromatography, Acetic acid, Angelica sinensis, Solvent, Methanol, Medicine