In vitro Regeneration and Its Influential Factors in Pepper (Capsicum annuum L.)

In vitro plant regeneration was achieved from eight pepper materials(Capsicum annuum L.).The effects of genotype,various explant types(cotyledons,hypocotyls) and hormones were evaluated.It was shown that the high 6-BA/IAA ratio was conducive to the differentiation and the low 6-BA/IAA ratio was suitable for the elongation of the regenerative buds;different pepper materials differed greatly in their regeneration capacities.The cotyledons had stronger regenerative capacity than that of the hypocotyls,and the explants from 12～16 day old seedlings had a high differentiation rate.Higher regeneration frequency was obtained from materials of '2096','B4' and 'B7'.The optimal medium for the differentiation of pepper buds was Murashige and Skoog(MS)medium supplemented with B5 organic nutrient(MB),0.8 mg/L IAA,5.0 mg/L 6-BA and 4.0 mg/L AgNO_3.The best media for shoots elongation was MB medium with 0.8 mg/L IAA,2.0 mg/L 6-BA,2.0 mg/L GA_3 and 4.0 mg/L AgNO_3 and the rooting medium was MB medium with 0.2 mg/L IAA,0.1 mg/L NAA.