Study on Improve Elongation Rate of Adventitious Bud Regeneration of Pepper (Capsicum annuum L.)

An efficient procedure to improve elongation rate of adventitious bud in vitro regeneration of Pepper was obtained by adjusting concentration and combinations of several plant growth regulators and some supplements.The cotyledons with petiole of 9-11-day-old seeding were cultured on MB(MS mineral+Vitamin B5)+BA 5.0 mg/L+IAA 1.0 mg/L+GA3 1.5 mg/L+sucrose 3%+agar 6.5 g/L+CM(coconut milk) 5%+AgNO3 5.0 mg/L.And the differentiation rate is 97.8%,with 1-2 adventitious bud rosettes per explants.Adventitious bud rosettes were elongated on MB+ZT 1.0 mg/L+IAA 1.0 mg/L+GA3 1.5 mg/L+CM(coconut milk) 5%+AgNO3 5.0 mg/L+sucrose 3%+agar 6.5 g/L medium,and the utmost rate of elongation is 76.0%.Rooting of the regenerated shoots was achieved on MS+IAA 0.1 mg/L+NAA 0.2 mg/L medium,and the rooting rate is 100%.It grew up to be strong regenerated plant at last.