Proliferation Model of Human Oral Mucosal Fibroblasis Induced by Extract from Areca Nut in Vitro

Objective To provide a proliferation model of human oral mucosal fibroblasts for the study of repair mechanism on oral submucous fibrosis.Methods Human oral mucosal fibroblasts were cultured in vitro and identified by vimentin and kieratin immunochemistry method;the culturing fibroblasts were induced by areca nut extract(ANE) with different concentration(0,5,50,100,150 and 200 μg/mL);and then the fibroblasts proliferation was detected with MTT assay.Results The expression of the vimentin was positive while the keratin negative,and ANE with concentration 50 μg/mL and 100 μg/mL significantly promoted the fibroblasts proliferation.Conclusion The cultured cells are purifying human oral mucosal fibroblasts,the appropriate concentration for ANE inducing proliferation of oral mucosal fibroblasts are 50 μg/mL-100 μg/mL.This model can be a basis for the farther study of oral submucous fibrosis repair mechanism.