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2006•Journal of Clinical StomatologyRequires access

Influence of oral keratinocytes stimulated by aqueous areca nut extract on proliferation of fibroblasts in vitro

Ling Tian-yo

Open publisher page 2 citations

Abstract

Objective:To evaluate the role of keratinocytes derived from oral mucosa in the development of oral submucous fibrosis(OSF) and to further elucidate the etiology and pathogenesis of OSF.Method: Human oral mucosa keratinocytes were isolated,cultured,stimulated with aqueous areca nut extract(ANE).The keratinocytes conditioned medium(KCM) after stimulated by ANE was aspirated and then the proliferation of human fibroblasts cultured in different concentration of KCM was detected with MTT method.Result:The KCM increased the proliferation of fibroblasts which showed indiviual diversity.Conclution:ANE may induce OSF through changing the biological activity of oral keratinocyte.

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What this paper is about

Objective:To evaluate the role of keratinocytes derived from oral mucosa in the development of oral submucous fibrosis(OSF) and to further elucidate the etiology and pathogenesis of OSF.Method: Human oral mucosa keratinocytes were isolated,cultured,stimulated with aqueous areca nut extract(ANE).The keratinocytes conditioned medium(KCM) after stimulated by ANE was aspirated and then the proliferation of human fibroblasts cultured in different concentration of KCM was detected with MTT method.Result:The KCM increased the proliferation of fibroblasts which showed indiviual diversity.Conclution:ANE may induce OSF through changing the biological activity of oral keratinocyte.

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Available abstract

Objective:To evaluate the role of keratinocytes derived from oral mucosa in the development of oral submucous fibrosis(OSF) and to further elucidate the etiology and pathogenesis of OSF.Method: Human oral mucosa keratinocytes were isolated,cultured,stimulated with aqueous areca nut extract(ANE).The keratinocytes conditioned medium(KCM) after stimulated by ANE was aspirated and then the proliferation of human fibroblasts cultured in different concentration of KCM was detected with MTT method.Result:The KCM increased the proliferation of fibroblasts which showed indiviual diversity.Conclution:ANE may induce OSF through changing the biological activity of oral keratinocyte.

Key concepts: Areca, Oral submucous fibrosis, Keratinocyte, Oral mucosa, In vitro, Fibroblast, Chemistry, Molecular biology

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