Inhibition of Danxuan Koukang on collagen synthesis and TGFβ_1 expression in human oral mucosal fibroblasts induced by areca nut extract

Objective To study the effect of prepared Danxuan Koukang(DXKK) drug serum on collagen synthesis and expression of transcausing growth factor β1(TGFβ1) in human oral mucosal fibroblasts(FB) induced by areca nut extract(ANE).Methods Human oral mucosal FB were cultured in vitro with inducement ANE and intervenor 10% DXKK drug serum;Collagen synthesiswas measured by 3H-proline;and TGFβ1 expression was examined by immunocytechemistry and ELISA.Results 3H-proline incorporation value showed that ANE could promote collagen synthesis of human oral mucosal FB,the promotion was restrained by DXKK drug serum in a manner of dose-effect relationship(P0.01 or P0.05);immunocytechemistry and ELISA showed that the TGFβ1 expression of human oral mucosal FB was depressed by DXKK drug serum(P0.01).Conclusions DXKK drug serum can inhibit the collagen synthesis stimulated by ANE through down-regulating TGFβ1 expression.