2004•Northwest Pharmaceutical JournalRequires access

Determination of berberine hydrochloride in coptis decoction by HPLC.

Qing Li

Abstract

Objective To determine the content of berberine hydrochloride in coptis decoction by RP-HPLC. Methods The samples were separated on a Hypersil C 18 column(200 mm×4.6 mm,5 μm)with acetonitrile-water-triethylamine(26∶74∶0.2) as the mobile phase. The UV detection was set at 340 nm. The flow rate was 0.8 mL/min. Results Thelinearcalibration curve was obtained in the concentrationrangeof3.54～17.68 mg·L -1. The mean recovery was 94.8% and RSD was 2.0%(n=9). Conclusion This method was simple, reliable and suitable for the quality control of berberine hydrochloride in coptis decoction.

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Key concepts: Berberine hydrochloride, Coptis, Decoction, Chemistry, Chromatography, Berberine, High-performance liquid chromatography, Coptisine