2004•ZhongchengyaoRequires access

Determination of berberine hydrochloride in Huanglianshangqing Tablets by HPLC

YU Shao-ting

Abstract

AIM: To establish HPLC method for the determination of berberine hydrochloride in Huanglianshangqing Tablets(Rhizoma Coptidis, Radix et Rhizoma Rhei, Fructus Forsythiae, etc.). METHODS: The Diamonsil TM C 18(20cm×4.6mm,5μm) was used with 0.033mol·L -1 Potassium dihydrogen phosphate solution-acetonitrile (70∶30) as a mobile phase. The flow rate was 1.0 mL·min -1. The detecting wavelength was 265nm. RESULTS: The linear of berberine hydrochloride was in the range of 0.2376～ 0.5544μg, r= 0.9999. The average recovery was 98.7% and RSD was 0.7%(n=5), respectively. CONCLUSION: The method is simple,rapid and accurate.

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Key concepts: Berberine hydrochloride, Chromatography, Chemistry, Berberine, High-performance liquid chromatography, Potassium, Radix (gastropod), Linear relationship