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2010•Northern HorticultureRequires access

Effects of Different Hormone Medium on Induction of The Callus of Chrysanthemum Leaves and Regeneration

Chong-Mei Zhu

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Abstract

Explants of Chrysanthemum leaves were cultured on the media of different phytohormone combinations.Based on 6-BA and NAA in the Chrysanthemum the role of tissue culture,stated in the hormone's role in the propagation of chrysanthemum.Choose a hormone levels were proper induced callus and cluster buds.The results indicated that MS+6-BA 2.0 mg/L+NAA 1.0 mg/L medium was the best to induce callus.The MS+6-BA 2.0～3.0 mg/L+NAA 0.1～0.5 mg/L medium was the best to induce cluster buds.The higher regeneration rate was got from MS medium with 6-BA 2 mg/L+NAA 0.1 mg/L.All tube shoots can root on the 1/2MS medium with 0.1 mg/L NAA +20 g/L sucrose.

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What this paper is about

Explants of Chrysanthemum leaves were cultured on the media of different phytohormone combinations.Based on 6-BA and NAA in the Chrysanthemum the role of tissue culture,stated in the hormone's role in the propagation of chrysanthemum.Choose a hormone levels were proper induced callus and cluster buds.The results indicated that MS+6-BA 2.0 mg/L+NAA 1.0 mg/L medium was the best to induce callus.The MS+6-BA 2.0～3.0 mg/L+NAA 0.1～0.5 mg/L medium was the best to induce cluster buds.The higher regeneration rate was got from MS medium with 6-BA 2 mg/L+NAA 0.1 mg/L.All tube shoots can root on the 1/2MS medium with 0.1 mg/L NAA +20 g/L sucrose.

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Available abstract

Explants of Chrysanthemum leaves were cultured on the media of different phytohormone combinations.Based on 6-BA and NAA in the Chrysanthemum the role of tissue culture,stated in the hormone's role in the propagation of chrysanthemum.Choose a hormone levels were proper induced callus and cluster buds.The results indicated that MS+6-BA 2.0 mg/L+NAA 1.0 mg/L medium was the best to induce callus.The MS+6-BA 2.0～3.0 mg/L+NAA 0.1～0.5 mg/L medium was the best to induce cluster buds.The higher regeneration rate was got from MS medium with 6-BA 2 mg/L+NAA 0.1 mg/L.All tube shoots can root on the 1/2MS medium with 0.1 mg/L NAA +20 g/L sucrose.

Key concepts: Callus, Sucrose, Explant culture, Shoot, Murashige and Skoog medium, Tissue culture, Regeneration (biology), Botany

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Effects of Different Hormone Medium on Induction of The Callus of Chrysanthemum Leaves and Regeneration — Research Paper | ScholarLens