Tissue Culture of Chrysanthemum Petal
Fanli Zeng
Abstract
Fanli Zeng
Abstract
In this experiment,sprouts are acquired through tissue culture of petals on chrysanthemum.The results of tissue culture under the influences of 6-BA and NAA were reported.Two kinds of approaches to get sprouts were obtained-the first approach was to induce callus,then buds on callus under the influences of MS+6-BA 1.0 mg/L+NAA 0.2 mg/L and then MS+6-BA 2.0 mg/L+NAA 0.1 mg/L;while the other approach is to lead buds directly cultured on MS medium supplemented with 2.0 mg/L 6-BA and 0.1 mg/LNAA for weeks.
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In this experiment,sprouts are acquired through tissue culture of petals on chrysanthemum.The results of tissue culture under the influences of 6-BA and NAA were reported.Two kinds of approaches to get sprouts were obtained-the first approach was to induce callus,then buds on callus under the influences of MS+6-BA 1.0 mg/L+NAA 0.2 mg/L and then MS+6-BA 2.0 mg/L+NAA 0.1 mg/L;while the other approach is to lead buds directly cultured on MS medium supplemented with 2.0 mg/L 6-BA and 0.1 mg/LNAA for weeks.
Key concepts: Petal, Callus, Tissue culture, Botany, Chemistry, Horticulture, Biology, In vitro