2007Northern HorticultureRequires access

Tissue Culture of Chrysanthemum Petal

Fanli Zeng

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Abstract

In this experiment,sprouts are acquired through tissue culture of petals on chrysanthemum.The results of tissue culture under the influences of 6-BA and NAA were reported.Two kinds of approaches to get sprouts were obtained-the first approach was to induce callus,then buds on callus under the influences of MS+6-BA 1.0 mg/L+NAA 0.2 mg/L and then MS+6-BA 2.0 mg/L+NAA 0.1 mg/L;while the other approach is to lead buds directly cultured on MS medium supplemented with 2.0 mg/L 6-BA and 0.1 mg/LNAA for weeks.

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What this paper is about

In this experiment,sprouts are acquired through tissue culture of petals on chrysanthemum.The results of tissue culture under the influences of 6-BA and NAA were reported.Two kinds of approaches to get sprouts were obtained-the first approach was to induce callus,then buds on callus under the influences of MS+6-BA 1.0 mg/L+NAA 0.2 mg/L and then MS+6-BA 2.0 mg/L+NAA 0.1 mg/L;while the other approach is to lead buds directly cultured on MS medium supplemented with 2.0 mg/L 6-BA and 0.1 mg/LNAA for weeks.

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Available abstract

In this experiment,sprouts are acquired through tissue culture of petals on chrysanthemum.The results of tissue culture under the influences of 6-BA and NAA were reported.Two kinds of approaches to get sprouts were obtained-the first approach was to induce callus,then buds on callus under the influences of MS+6-BA 1.0 mg/L+NAA 0.2 mg/L and then MS+6-BA 2.0 mg/L+NAA 0.1 mg/L;while the other approach is to lead buds directly cultured on MS medium supplemented with 2.0 mg/L 6-BA and 0.1 mg/LNAA for weeks.

Key concepts: Petal, Callus, Tissue culture, Botany, Chemistry, Horticulture, Biology, In vitro

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