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Research on Using Cotolydon as Explant in Vitro for Regeneration in Cucumber

Yuejin Xu

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Abstract

Effects of different plant hormone on callus induction and callus growth in cucumber were investigated by Orthogonal design. The main results that the medium for callus induction was MS+1.0 mg/L 6-BA+(1.0～1.5) mg/L KT+0.3 mg/L NAA+0.1 mg/L 2,4-D. The results also showed that the medium for callus growth was MS+6-BA1.5 mg/L+ NAA 0.3 mg/L. The adventitious shoots were placed on medium (MS+0.1 mg/L NAA) for root regeneration. The whole regenerated plants could be obtained in 40~60 days. In addition, some adventitious-buds were accompanied with the growth of callus.

About this research paper

What this paper is about

Effects of different plant hormone on callus induction and callus growth in cucumber were investigated by Orthogonal design. The main results that the medium for callus induction was MS+1.0 mg/L 6-BA+(1.0～1.5) mg/L KT+0.3 mg/L NAA+0.1 mg/L 2,4-D. The results also showed that the medium for callus growth was MS+6-BA1.5 mg/L+ NAA 0.3 mg/L. The adventitious shoots were placed on medium (MS+0.1 mg/L NAA) for root regeneration. The whole regenerated plants could be obtained in 40~60 days. In addition, some adventitious-buds were accompanied with the growth of callus.

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Available abstract

Effects of different plant hormone on callus induction and callus growth in cucumber were investigated by Orthogonal design. The main results that the medium for callus induction was MS+1.0 mg/L 6-BA+(1.0～1.5) mg/L KT+0.3 mg/L NAA+0.1 mg/L 2,4-D. The results also showed that the medium for callus growth was MS+6-BA1.5 mg/L+ NAA 0.3 mg/L. The adventitious shoots were placed on medium (MS+0.1 mg/L NAA) for root regeneration. The whole regenerated plants could be obtained in 40~60 days. In addition, some adventitious-buds were accompanied with the growth of callus.

Key concepts: Callus, Explant culture, Regeneration (biology), Shoot, Murashige and Skoog medium, Botany, In vitro, Plant growth

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