Effect of "Lanyin Fengyang Huazhuo Jiedu Decoction" on expressions of TNF-α,TLR_4 and NF-kBp65 in colonic mucosa in ulceratvie colitis rats

Objective To observe the effect of Lanyin Fengyang Huazhuo Jiedu Decoction on expressions of TNF-α,TLR4 and NF-kBp65 in colonic mucosa in ulceratvie colitis rats.Methods Sixty eight-week-old healthy male Wistar rats were randomly divided into 5 groups with 12 rats in each group:normal group(NG),model group(MG),high-dose group ofLanyin Fengyang Huazhuo Jiedu Decoction(HDG),low-dose group ofLanyin Fengyang Huazhuo Jiedu Decoction(LDG) and SASP group(SPAP).Trini-trobenzenesul-fonic acid/ethanol(TNBs/ethanol) was used to induce ulceratvie colitis.After modeling,the rats in the five groups were treated respectively.The rats’ general situation,DAI scores,histopathological changes,TNF-α in serologic test,TLR4 and NF-kBp65 were observed with immunohistochemistry.Results Compared with NG,DAI scores and the expressions of TNF-α,TLR4 and NF-kBp65 in MG were statistically significant(P0.05);HDG and LDG vs MG,the differences were all statistically significant(P0.05);HDG vs LDG,the difference was statistically significant(P0.05);HDG vs SASP,the difference was statistically significant(P0.05).LDG vs SASP,the difference was not statistically significant(P0.05).Conclusion Lanyin Fengyang Huazhuo Jiedu Decoctionhas good results on ulceratvie colitis with dose-effect dependent relation by regulating TLRs/NF-kB pathway through TNF-α and inhibiting NF-kB activation and release to fight inflammation and adjust body immunity.