Effect of Xiezhuo Jiedu decoction on expression of ICAM-1 of rat with chronic nonspecific ulcerative colitis

Objective To study the effect of Xiezhuo Jiedu decoction on expression of ICAM-1 of rat with chronic nonspecific ulcerative colitis induced by trinitrobenzene sulfonic acid(TNBS).Methods Ulcerative colitis rats were induced by intragastric administration of trinitrobenzene sulfonic acid(TNBS).40 SD rats were randomly divided into 4 groups:model group,Xiezhuo Jiedu Decoction high dose group,Xiezhuo Jiedu Decoction low dose group,positive control group,and 10 rats were chosen to be blank control group before model making.The rats in all the groups were administered by intragastric administration.The rats in model group and blank control group were administered by 0.9% sodium chloride injection,the rats in positive control group were given Sulfasalazine(0.30 g/kg),the rats in Xiezhuo Jiedu Decoction high dose group and Xiezhuo Jiedu Decoction low dose group were given Xiezhuo Jiedu Decoction stock solution(4 mL/d,1 mL/d),all once a day.Intragastric administration was begun 3days after model-making and lasted 14 days.18 days after model-making,the changes of morphology and histology of colonic mucosa,disease active index(DAI) and the rats'ICAM-1 were observed in all the groups.Result The scores of DAI and ICAM-1 in positive control group,Xiezhuo Jiedu Decoction high dose group,and Xiezhuo Jiedu Decoction low dose group were decreased in comparison with those in control group(P 0.01).The change of DAI in Xiezhuo Jiedu Decoction high dose group was superior to that in Xiezhuo Jiedu Decoction low dose group and positive control group(P 0.05,P 0.01).The score of ICAM-1 Xiezhuo Jiedu Decoction high dose group and Xiezhuo JieduDecoction low dose group was lower than that in positive control group(P 0.05).There was no significant difference between Xiezhuo Jiedu Decoction high dose group and Xiezhuo Jiedu Decoction low dose group on the score of ICAM-1(P 0.05).Conclusion Xiezhuo Jiedu Decoction has definite effect on ulcerative colitis rats induced by trinitrobenzene sulfonic acid(TNBS),and Xiezhuo Jiedu Decoction high dose group is superior to positive control group.The mechanism of action was related to down-regulate the expression of ICAM-1.