Effect of Lanyin Fengyang Huazhuo Jiedu Prescription on Immune Regulation Mechanism in Rats with Ulcerative Colitis

Objective:To observe the effect of Lanyin Fengyang Huazhuo Jiedu prescription on expressions of CD4+/CD29+,IL-8 and IL-10 in rats with ulcerative colitis.Method:Sixty healthy male Wistar rats were randomly divided into 5 groups:the normal group(NG),model control group(MG),high-dose group of Lanyin Fengyang Huazhuo Jiedu decoction(HDG,20.0 g·kg-1),low-dose group of Lanyin Fengyang Huazhuo Jiedu decoction(LDG,5.0 g·kg-1) and the sulfasalazine group(SPAP 0.3 g·kg-1),12 rats in every group.Trinitrobenzenesu l-fonicacid/ethanol(TNBs/ethanol) was used to induce ulceratvie colitis.After successful modeling,continuous treatment lasted 14 days.The rat's general state,disease activity index(DAI) scores,colonic pathological morphology change of T-lymphocyte cytokines IL-8 and IL-10 were observed.Result:DAI scores:compared with NG,the scores of other groups were elevated.compared with the MG,the scores of HDG and LDG were reduced.The result showed statistical significance(P0.05).CD4+,CD29+:compared with the MG,the levels of NG,HDG and LDG were reduced.The result showed statistical significance(P0.05).IL-8:compared with the MG,the levels of NG,HDG and LDG were reduced(P0.05).There was no difference between the levels of LDG and SG.IL-10:compared with the MG,the levels of SPAP,HDG and LDG were elevated(P0.05).Conclusion:Lanyin Fengyang Huazhuo Jiedu decoction can raise the suppression of inflammatory cytokines,reduce promoting the inflammatory factor and suppressioned of CD4+/CD29+expression.