Determination of benzoquanamine in aqueous food simulants of food contact materials by high-performance liquid chromatography

Objective:To develop a method for the determination of benzoquanamine in aqueous food simulants of food contact materials by using high-performance liquid chromatography.Methods:The samples were extracted by distilled water,10%(v/v) ethanol aqueous solution,20%(v/v) ethanol aqueous solution,50%(v/v) ethanol aqueous solution and 3%(w/v) acetic acid aqueous solution.The extract was separated on a Agilent HC-CN analytical column with a mixture of acetonitrile-water(18:82,by volume) as mobile phase,and detected by ultraviolet detector at a wavelength of 246 nm.Results:The limit of detection(S/N = 3) for benzoquanamine in five aqueous food simulants was was 0.031 mg/L～0.046 mg/L with a good linear correlation(r≥0.9998) in the range of 0.5 mg/L～10.0 mg/L.The mean recovery of the compound at three different spiked levels were 82.0%～97.3%,with the relative standard deviation(n = 6) of 0.81%～5.9%.Conclusion:The method was simple,rapid,accurate and it can satisfy the inspection of benzoquanamine in food contact materials.