Determination of melamine in aqueous food simulants of melamine articles by high-performance liquid chromatography

Objective:To delevop a method for the determination of melamine in aquesous food simulants of melamine articles by using high-performance liquid chromatography.Methods:After being extracted by distilled water,10%(v/v)ethanol aqueous solution and 3%(w/v)acetic acid aqueous solution from samples,melamine was seperated on a C18 analytical column with acetonitrile and sodium 1-heptanesulfonate(0.01 mol/L,pH3.1,10:90 by volume) detected by ultraviolet detector at a wavelength of 236 nm.Results:The linear ranges of determination of melamine in distilled water,10%(v/v)ethanol aqueous solution and 3%(w/v)acetic acid aqueous solution were 1.0~20.0 mg/L,the correlation coefficent was 0.9999,0.9998,0.9999 respectively,the detection of limit(S/N=3) was 0.04,0.04,0.05 mg/L respectively,the relative standard deviation(n=6)was 1.9%~6.6%.Conclusion:The method has the advatages of simplicity,convenience and repeatablity and can be used for the analysisi of melamine in aqueous food simulants of melamine articles.