Determination of 2,4-dihydroxy benzophenone in aqueous food simulants of food contact materials by high-pressure liquid chromatography

Objective To develop a method for determination of 2,4-dihydroxy benzophenone in aqueous food simulants of food contact materials by high-pressure liquid chromatography. Methods Samples were extracted by distilled water, 10%(v/v) ethanol aqueous solution, 20% (v/v) ethanol aqueous solution, 50% (v/v) ethanol aqueous solution and 3% (w/v) acetic acid aqueous solution. The extracts were separated on an Agilent HC-CN analytical column with a mixture of methanol-water (55: 45, v/v) as mobile phase and detected by an ultraviolet detector at a wavelength of 290 nm. Results The limit of detection (S/N=3) for 2,4-dihydroxy benzophenone in 5 aqueous food simulants was 0.02 mg/L with a good linear correlation (r=0.9999) in the range of 0.5~10.0 mg/L. The mean recovery of the compound at 3 different spiked levels was 84.0%~96.9%, with the relative standard deviation (n=6) 0.85%~4.60%. Conclusion The method is simple, rapid and accu-rate, which could be used in the detection of 2,4-dihydroxy benzophenone in food contact materials.