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Determination of oxymatrine in Herihu-5 by RP-HPLC

Chunjie Zhao, Rina Chin, R China

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Abstract

OBJECTIVE To establish an HPLC method for the determination of oxymatrine in Herihu-5.METHODS Seperation was achieved on a Shim-pack CLC-ODS column and detected at 210 nm.The mobile phase was the mixture of methanol and 0.1% triethylamine(3∶8,adjust pH3.0 with 10% phosphoric acid) at a flow rate of 1.0 mL·min-1.RESULTS The linear range was 4.1– 41 mg·L-1.The linear equation was Y=36.804X + 0.4436(r=0.9999).The mean method recovery was 99.4% with RSD of 1.4%(n=6).CONCLUSION The method is simple,rapide,accurate.It is suitable for the determination of oxymatrine in Herihu-5.

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What this paper is about

OBJECTIVE To establish an HPLC method for the determination of oxymatrine in Herihu-5.METHODS Seperation was achieved on a Shim-pack CLC-ODS column and detected at 210 nm.The mobile phase was the mixture of methanol and 0.1% triethylamine(3∶8,adjust pH3.0 with 10% phosphoric acid) at a flow rate of 1.0 mL·min-1.RESULTS The linear range was 4.1– 41 mg·L-1.The linear equation was Y=36.804X + 0.4436(r=0.9999).The mean method recovery was 99.4% with RSD of 1.4%(n=6).CONCLUSION The method is simple,rapide,accurate.It is suitable for the determination of oxymatrine in Herihu-5.

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Available abstract

OBJECTIVE To establish an HPLC method for the determination of oxymatrine in Herihu-5.METHODS Seperation was achieved on a Shim-pack CLC-ODS column and detected at 210 nm.The mobile phase was the mixture of methanol and 0.1% triethylamine(3∶8,adjust pH3.0 with 10% phosphoric acid) at a flow rate of 1.0 mL·min-1.RESULTS The linear range was 4.1– 41 mg·L-1.The linear equation was Y=36.804X + 0.4436(r=0.9999).The mean method recovery was 99.4% with RSD of 1.4%(n=6).CONCLUSION The method is simple,rapide,accurate.It is suitable for the determination of oxymatrine in Herihu-5.

Key concepts: Oxymatrine, Chemistry, Chromatography, Phosphoric acid, Triethylamine, Linear range, High-performance liquid chromatography, Methanol

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