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Effects of different hormone levels on tissue culture of Anoectochilus roxburghii

Duan Chun-hong

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Abstract

Effects of different hormone on tissue culture of Anoectochilus roburghii were studied using stem tablets,stem segments and young shoots as explants respectively.The results indicated that the optimized medium of protocorm-like bodies(PLB) induction was MS +6-BA 2.0 mg/L +2,4-D 0.2 mg/L +ZT 0.2 mg/L +sucrose 30 g/L +agar 7 g/L;the appropriate medium for induction and proliferation of adventitious bud was MS +6-BA 2.0 mg/L +NAA 0.5 mg/L +TDZ 0.2 mg/L +sucrose 30 g/L +agar 7 g/L and the proliferation rate reached 23-fold after 90 days.The best rooting medium was 1/2MS+NAA 0.5 mg/L+IBA 4.0 mg/L+6-BA 0.1 mg/L+ sucrose 30 g/L+sugar 7 g/L+AC 3 g/L+banana juice 100 g/L.

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Effects of different hormone on tissue culture of Anoectochilus roburghii were studied using stem tablets,stem segments and young shoots as explants respectively.The results indicated that the optimized medium of protocorm-like bodies(PLB) induction was MS +6-BA 2.0 mg/L +2,4-D 0.2 mg/L +ZT 0.2 mg/L +sucrose 30 g/L +agar 7 g/L;the appropriate medium for induction and proliferation of adventitious bud was MS +6-BA 2.0 mg/L +NAA 0.5 mg/L +TDZ 0.2 mg/L +sucrose 30 g/L +agar 7 g/L and the proliferation rate reached 23-fold after 90 days.The best rooting medium was 1/2MS+NAA 0.5 mg/L+IBA 4.0 mg/L+6-BA 0.1 mg/L+ sucrose 30 g/L+sugar 7 g/L+AC 3 g/L+banana juice 100 g/L.

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Available abstract

Effects of different hormone on tissue culture of Anoectochilus roburghii were studied using stem tablets,stem segments and young shoots as explants respectively.The results indicated that the optimized medium of protocorm-like bodies(PLB) induction was MS +6-BA 2.0 mg/L +2,4-D 0.2 mg/L +ZT 0.2 mg/L +sucrose 30 g/L +agar 7 g/L;the appropriate medium for induction and proliferation of adventitious bud was MS +6-BA 2.0 mg/L +NAA 0.5 mg/L +TDZ 0.2 mg/L +sucrose 30 g/L +agar 7 g/L and the proliferation rate reached 23-fold after 90 days.The best rooting medium was 1/2MS+NAA 0.5 mg/L+IBA 4.0 mg/L+6-BA 0.1 mg/L+ sucrose 30 g/L+sugar 7 g/L+AC 3 g/L+banana juice 100 g/L.

Key concepts: Sucrose, Agar, Explant culture, Tissue culture, Shoot, Chemistry, Sugar, Botany

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