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Study on the Technology Isolated Culture and Rapid Propagation of Tomato

Zhuying Li

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Abstract

Tomato seeds chosen as test material,by the method of orthogonal test of preparation of different media,the effect of different concentrations of 6-BA,NAA,IBA on adventitious bud induction and proliferation of multiple shoot clumps were studied.The results showed that optimum medium for callus induction was MS+6-BA 2.0 mg/L+ IBA 0.3 mg/L+sucrose 30 g/L;optimum medium for adventitious bud induction was MS+6-BA 3.0 mg/L+NAA 0.1 mg/L+sucrose 30 g/L;suitable medium for the proliferation buds was MS+6-BA 1.0 mg/L+NAA 0.01 mg/L+sucrose 30 g/L;suitable medium for rooting medium was 1/2MS+NAA 0.6 mg/L+sucrose 30 g/L.

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What this paper is about

Tomato seeds chosen as test material,by the method of orthogonal test of preparation of different media,the effect of different concentrations of 6-BA,NAA,IBA on adventitious bud induction and proliferation of multiple shoot clumps were studied.The results showed that optimum medium for callus induction was MS+6-BA 2.0 mg/L+ IBA 0.3 mg/L+sucrose 30 g/L;optimum medium for adventitious bud induction was MS+6-BA 3.0 mg/L+NAA 0.1 mg/L+sucrose 30 g/L;suitable medium for the proliferation buds was MS+6-BA 1.0 mg/L+NAA 0.01 mg/L+sucrose 30 g/L;suitable medium for rooting medium was 1/2MS+NAA 0.6 mg/L+sucrose 30 g/L.

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Available abstract

Tomato seeds chosen as test material,by the method of orthogonal test of preparation of different media,the effect of different concentrations of 6-BA,NAA,IBA on adventitious bud induction and proliferation of multiple shoot clumps were studied.The results showed that optimum medium for callus induction was MS+6-BA 2.0 mg/L+ IBA 0.3 mg/L+sucrose 30 g/L;optimum medium for adventitious bud induction was MS+6-BA 3.0 mg/L+NAA 0.1 mg/L+sucrose 30 g/L;suitable medium for the proliferation buds was MS+6-BA 1.0 mg/L+NAA 0.01 mg/L+sucrose 30 g/L;suitable medium for rooting medium was 1/2MS+NAA 0.6 mg/L+sucrose 30 g/L.

Key concepts: Sucrose, Callus, Shoot, Tissue culture, Murashige and Skoog medium, Chemistry, Botany, Horticulture

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