Construction of the Regeneration System for in Vitro Culture of Photinia×frasery

Photinia×frasery stem and leaves were used as the explants to study the effects of external hormone on the buds induction, differentiation, multiplication and roots formation.The results showed that in the medium of MS+6-BA 3.0 mg/L+ NAA 0.3 mg/L+GA 0.5 mg/L+ sucrose 30 g/L+agar 7g/L, the percentage of induction was 63.3%; In the medium of MS+6-BA 0.5 mg/L+2,4-D 1.0 mg/L+NAA 1.0 mg/L+sucrose 30g/L+agar 7g/L, the calli forming rate was 86.0%; In the medium of N6+6-BA 1.5 mg/L+IAA 0.1 mg/L+sucrose 30g/L+agar 7g/L, the percentage of induction was 21.6%, multiplication times 1.4; In the medium of MS+6-BA 3.0 mg/L+KT 0.5 mg/L+NAA 0.3 mg/L+GA 0.7 mg/L+sucrose 30g/L+agar 7g/L, the multiplication times were 6.1; Explants soaked for 0.5～1.0 h after the 25mg/L NAA solution to vaccinate in the 1/2MS medium, the radication rate was more than 93.4%, using stroma of peat: perlite is 3∶1 to acclimate the test-tube plantlets, the survival rate was 96.7%.