Optimizing for callus tissue culture system for the stem of Solanum tuberosum

Four potato cultivars,Kexin No.12,Kexin No.13,Dongnong303 and Zaodabai were cultivated in vitro from stem explants.The results showed that the optimal medium of callus for Kexin No.12 and Kexin No.13 was MS+30 g/L sucrose+8g/L agar +2 mg/L6-BA+1 mg/L 2,4-D;Dongnong 303 was MS+2 mg/L 6-BA +0.5 mg/L 2,4-D;the optimal medium of Zaodabai was MS+30 g/L sucrose+8 g/L agar+2 mg/L 6-BA+0.5 mg/L 2,4-D;the adventitious callus induction rate were 100%,100%,100% and 90%,respectively by using these optimal induced medium.The best medium for adventitious bud differentiation from stem callus was MS+30 g/L sucrose+8 g/L agar+4.0 mg/L 6-BA+0.1 mg/L NAA+1.0 mg/L GA3 for Kexin13;MS+30 g/L sucrose+8 g/L agar+2.0 mg/L 6-BA+0.5 mg/L NAA+1.0 mg/L GA3 for Kexin12;MS+30 g/L sucrose+8 g/L agar+2.0 mg/L 6-BA+0.1 mg/L NAA+1.5 mg/L GA3 for Dongnong 303;MS+30 g/L sucrose+8 g/L agar+2.0 mg/L 6-BA+0.5 mg/L NAA+1.0 mg/L GA3 for Zaodabai.Under these optimal induced medium,the rates of adventitious bud differentiation were 80%,90%,100%,and 76.7%,respectively.